Bacterial Genome Sequencing?

[noah]

Anyone know how long it takes to sequence an entire bacterial genome using standard lab equipment?

[liam]

Can you clarify what you mean by standard lab equipment? Standard for a facility that sequences genomes is nothing like standard lab equipment in a wet-lab.

[noah]

standard lab equipment (running sequencing gels )

[liam]

@noah
The sequencing never took long, usually around 9-12 hours depending on how long the sequence was. The problem with our stuff was, if you didn’t get everything exactly right, you’d be missing a few nucleotides here and there

[johnbelley]

I think you need to know how many manhours since it can be done by several groups at once; also it depends on how big the genome is (in base pairs).

[liam]

also you can Google “bacteria genome length” and “sequencing manhours per base pair” and do the math

[alex]

Couple days depending on how fast you get your results back after you do all the experimentation parts.

[Author]

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